LOCUS       V008093                 5883 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008093
VERSION     V008093
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Akagi T, Shishido T, Murata K, Hanafusa H.
  TITLE     v-Crk activates the phosphoinositide 3-kinase/AKT pathway in
            transformation
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (13), 7290-7295 (2000)
   PUBMED   10852971
REFERENCE   2  (bases 1 to 5883)
  AUTHORS   Akagi T.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-2000) Tsuyoshi Akagi, Osaka Bioscience Institute,
            The First Department; 6-2-4 Furuedai, Suita, Osaka 565-0874, Japan
            (E-mail:takagi@obisun1.obi.or.jp, Tel:81-6-6872-4850,
            Fax:81-6-6871-6686)
REFERENCE   3  (bases 1 to 5883)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5883)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2000"; volume: "97"; issue: "13"; pages:
            "7290-7295"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (17-APR-2000) Tsuyoshi Akagi, Osaka Bioscience Institute, The First
            Department"; volume: " 6-2-4 Furuedai, Suita, Osaka 565-0874, Japan
            (E-mail:takagi@obisun1.obi.or.jp, Tel:81-6-6872-4850, Fax"; pages:
            "81-6-6871-6686"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5883
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        88..467
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     repeat_region   467..762
                     /label="R-U5 region of MuLV 5'LTR"
                     /note="R-U5 region of MuLV 5'LTR"
     misc_feature    826..1183
                     /label="MMLV Psi"
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1248..1664
                     /label="gag (truncated)"
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     misc_feature    1674..2048
                     /label="pol region"
                     /note="Moloney murine leukemia virus (MMLV) pol region
                     containing the splice acceptor site"
     misc_feature    2052..2136
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     misc_feature    2185..2765
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             2765..3184
                     /gene="bsr"
                     /label="Blasticidin-S deaminase"
                     /note="Blasticidin-S deaminase from Bacillus cereus.
                     Accession#: P33967"
     LTR             3240..3833
                     /label="LTR"
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     rep_origin      complement(4070..4658)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4825..5682)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5683..5787)
                     /label="AmpR promoter"
ORIGIN
        1 tcgaggagct tggcccattg catacgttgt atccatatca taatatgtac atttatattg
       61 gctcatgtcc aacattaccg ccatgttgac attgattatt gactagttat taatagtaat
      121 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      181 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      241 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gactatttac
      301 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      361 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      421 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatgcta gagaaccatc
      481 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca
      541 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc
      601 cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt
      661 atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt
      721 ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttgggggc tcgtccggga
      781 tcgggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac
      841 ttatctgtgt ctgtccgatt gtctagtgtc tatgactgat tttatgcgcc tgcgtcggta
      901 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttcggaac
      961 acccggccgc aaccctggga gacgtcccag ggacttcggg ggccgttttt gtggcccgac
     1021 ctgagtccaa aaatcccgat cgttttggac tctttggtgc acccccctaa taggagggat
     1081 atgtggttct ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc
     1141 tttcggtttg ggaccgaagc cgcgccgcgc gtcttgtctg ctgcagcatc gttctgtgtt
     1201 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatat gggcccgggc cagactgtta
     1261 ccactccctt aagtttgacc ttaggtcact ggaaagatgt cgagcggatc gctcacaacc
     1321 agtcggtaga tgtcaagaag agacgttggg ttaccttctg ctctgcagaa tggccaacct
     1381 ttaacgtcgg atggccgcga gacggcacct ttaaccgaga cctcatcacc caggttaaga
     1441 tcaaggtctt ttcacctggc ccgcatggac acccagacca ggtcccctac atcgtgacct
     1501 gggaagcctt ggcttttgac ccccctccct gggtcaagcc ctttgtacac cctaagcctc
     1561 cgcctcctct tcctccatcc gccccgtctc tcccccttga acctcctcgt tcgaccccgc
     1621 ctcgatcctc cctttatcca gccctcactc cttctctagg cgcccccata tggccatatg
     1681 agatcttata tggggcaccc ccgccccttg taaacttccc tgaccctgac atgacaagag
     1741 ttactaacag cccctctctc caagctcact tacaggctct ctacttagtc cagcacgaag
     1801 tctggagacc tctggcggca gcctaccaag aacaactgga ccgaccggtg gtacctcacc
     1861 cttaccgagt cggcgacaca gtgtgggtcc gccgacacca gactaagaac ctagaacctc
     1921 gctggaaagg accttacaca gtcctgctga ccacccccac cgccctcaaa gtagacggca
     1981 tcgcagcttg gatacacgcc gcccacgtga aggctgccga ccccgggggt ggaccatcct
     2041 ctagactgcc ggatcccagt gtggtggtag ggaattcgag ctcggtaccc gggggcgcgc
     2101 cggatcctta attaagcggc cgcggtaaca attgttaact aactaactaa gctagcaacg
     2161 gtttccctct agcgggatca attccccctc tccctccccc ccccctaacg ttactggccg
     2221 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     2281 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     2341 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     2401 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     2461 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     2521 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     2581 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     2641 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     2701 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg ataataccat
     2761 ggtcatgaaa acatttaaca tttctcaaca agatctagaa ttagtagaag tagcgacaga
     2821 gaagattaca atgctttatg aggataataa acatcatgtg ggagcggcaa ttcgtacgaa
     2881 aacaggagaa atcatttcgg cagtacatat tgaagcgtat ataggacgag taactgtttg
     2941 tgcagaagcc attgcgattg gtagtgcagt ttcgaatgga caaaaggatt ttgacacgat
     3001 tgtagctgtt agacaccctt attctgacga agtagataga agtattcgag tggtaagtcc
     3061 ttgtggtatg tgtagggagt tgatttcaga ctatgcacca gattgttttg tgttaataga
     3121 aatgaatggc aagttagtca aaactacgat tgaagaactc attccactca aatatacccg
     3181 aaattaaaag tcgacacgat aaaataaaag attttattta gtctccagaa aaagggggga
     3241 atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt ttggaaggca
     3301 tggaaaaata cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca
     3361 gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     3421 agaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
     3481 cccggctcag ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta
     3541 gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt
     3601 gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa
     3661 taaaagagcc cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg
     3721 gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc
     3781 ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc attctgcatt
     3841 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     3901 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     3961 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     4021 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     4081 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4141 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4201 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     4261 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4321 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     4381 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4441 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     4501 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     4561 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     4621 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     4681 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     4741 caaaaaggat cttcacctag atccttttgc ggccggccgc aaatcaatct aaagtatata
     4801 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     4861 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     4921 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     4981 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     5041 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     5101 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     5161 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     5221 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     5281 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     5341 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     5401 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca
     5461 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     5521 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     5581 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     5641 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     5701 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     5761 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     5821 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     5881 tcc
//
